Summary:
The development of protocols for efficient approaches to cloning, expression, purification and crystallization of large numbers of tagged recombinant proteins is essential to shorten the time from gene to drug target. Automation increases throughput at each stage, and enables methods to be performed with a high level of robustness – increasing accaracy, enabling hands-off operation and eliminating human error – to give uncompromising experimental reproducibility and minimal variation among replicates.
challenges in protein expression screening, with examples that show how the combination of our products and expertise can help you.
? Multi-parallel screening of tagged proteins with high reproducibility and minimal cross-contamination.
? Screening of buffer conditions to optimize the recovery of a target protein, and using the identified conditions to scale up the purification.
? Identification of protein construct variants that improve crystallization propensity.
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