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| 中文名稱 | 絲氨酸蛋白酶8抗體 |
| 別????名 | CAP1; Channel Activating Protease 1; mCAP1; Prostasin; Prostasin heavy chain; protease, serine, 8; Prss8; PRSS8_HUMAN; Serine protease 8.?? |
| 研究領(lǐng)域 | 腫瘤??細(xì)胞生物??激酶和磷酸酶??腫瘤細(xì)胞生物標(biāo)志物?? |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,?Mouse,?Rat,? (predicted: Dog,?Pig,?Cow,?) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 30/33kDa |
| 細(xì)胞定位 | 細(xì)胞膜?細(xì)胞外基質(zhì)?分泌型蛋白? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human PRSS8:65-165/343? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ)?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | PRSS8 is a member of the trypsin family of serine proteases. It can be found in seminal fluid and is highly expressed in prostate epithelia. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. The carboxyterminal region contains a GPI anchor sequence, which anchors prostasin to the cell surface. Phospholipase activity is thought to liberate prostasin from the cell, and cleavage of the linker region also releases prostasin. PRSS8 has been proposed as a marker for ovarian cancer, where it is up-regulated, a marker for androgen-refractory prostate cancer, where it is down-regulated, and as an inhibitor of invasiveness in breast cancer. Function: Possesses a trypsin-like cleavage specificity. Subunit: Heterodimer of two chains, light and heavy, held by a disulfide bond. Subcellular Location: Prostasin: Cell membrane; Single-pass membrane protein. Prostasin light chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. Prostasin heavy chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. Tissue Specificity: Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: Q16651 Gene ID: 5652 Database links: Entrez Gene: 5652?Human Entrez Gene: 76560?Mouse Entrez Gene: 192107?Rat Omim: 600823?Human SwissProt: Q16651?Human SwissProt: Q9ESD1?Mouse SwissProt: Q9ES87?Rat Unigene: 75799?Human Unigene: 5875?Mouse Unigene: 107364?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產(chǎn)品圖片 |  Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti-PRSS8 (bs-6064R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/33 kD Observed band size: 40/43 kD  Sample:Lane 1: Muscle (Mouse) Lysate at 40 ug Lane 2: Muscle (Rat) Lysate at 40 ug Primary: Anti- PRSS8 (bs-6064R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37-40 kD Observed band size: 40 kD  Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated(bs-6064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated(bs-6064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |