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Entrez Gene: 5606?Human Entrez Gene: 26397?Mouse Entrez Gene: 303200?Rat Omim: 602315?Human SwissProt: P46734?Human SwissProt: O09110?Mouse Unigene: 514012?Human Unigene: 18494?Mouse Unigene: 100064?Rat
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中文名稱
磷酸化絲裂原活化蛋白激酶MKK3/6抗體
別????名
MKK3(Phospho-Ser218); MAP Kinase Kinase 3; Dual specificity mitogen activated protein kinase kinase 3; ERK kinase 3; MAP kinase kinase 3; MAP2K 3; MAPK ERK kinase 3; MAPK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK 3b; mMKK3b; MPK 3; PRKMK 3; PRKMK3; Protein kinase mitogen activated kinase 3; SKK2; zMKK 3; MP2K3_HUMAN; MP2K6_HUMAN??
產品類型
磷酸化抗體?
研究領域
腫瘤??信號轉導??細胞凋亡??生長因子和激素??轉錄調節因子??激酶和磷酸酶??細胞膜受體??
抗體來源
Rabbit
克隆類型
Polyclonal
交叉反應
Mouse,?Rat,? (predicted: Human,?Chicken,?Dog,?Pig,?Cow,?Horse,?Rabbit,?)
產品應用
WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量
39kDa
細胞定位
細胞核?細胞漿?細胞膜?
性????狀
Liquid
濃????度
1mg/ml
免?疫?原
KLH conjugated synthesised phosphopeptide derived from human MKK3 around the phosphorylation site of Ser218:VD(p-S)VA?
亞????型
IgG
純化方法
affinity purified by Protein A
儲?存?液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed
PubMed
產品介紹
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Function:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Subunit:
Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ.
Tissue Specificity:
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Post-translational modifications:
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE:
Note=Defects in MAP2K3 may be involved in colon cancer.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
P46734
Gene ID:
5606
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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產品圖片
Sample:
Muscle(Rat) Lysate at 40 ug
Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (bs-3274R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (bs-3274R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MKK3 (Ser218)+MKK6 (Ser207) Polyclonal Antibody, Unconjugated(bs-3274R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
磷酸化絲裂原活化蛋白激酶MKK3/6抗體
作者：上海雅吉生物科技有限公司 2020-10-29T00:00 (訪問量:5682)
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