?New epitope tag antibody (BirA (mutated/TurboID) ) for protein interaction studies?
Proximity labelling?is a laboratory technique which makes it possible to label proteins (in this case by biotinylation), proximal to protein of interest. This allows studying protein interactome networks in different types of cells and organs, as well as different developmental stages. The technique is achieved by a gene fusion in a living cell, between the protein of interest and BioID (BirA). The TurboID variant is a biotin ligase, with substantially higher activity, which provides higher temporal resolution and broader application?in vivo.?The labelling time is just 10 minutes in RT, compared to the over 18 hours of labelling time required for BioID.? anti-BirA (mutated/TurboID)? The proximity labelling technique is described in various publications, e.g.:? Zhang?et al. (2020). TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks.J Vis Exp. 2020 May 17;(159).doi: 10.3791/60728.? Cho?et al. (2020). Proximity labeling in mammalian cells with TurboID and split-TurboID.Nat Protoc. 2020 Dec;15(12):3971-3999. doi: 10.1038/s41596-020-0399-0.? Mair?et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in?Arabidopsis?enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. |
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