AANAT Knockout Lentivirus(AANAT基因敲除慢病毒)是一種感染動(dòng)物細(xì)胞后可以同時(shí)表達(dá)Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本產(chǎn)品用于在動(dòng)物細(xì)胞中基于CRISPR/Cas9技術(shù)敲除目的基因，并且本慢病毒中sgRNA的有效性已經(jīng)通過T7EI法的驗(yàn)證。
本慢病毒基因序列的關(guān)鍵圖譜信息請(qǐng)參考圖1。本慢病毒可用于感染細(xì)胞或組織并進(jìn)行目的基因的CRISPR/Cas9敲除。
圖1. 可同時(shí)表達(dá)sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的關(guān)鍵圖譜信息。
用于包裝本慢病毒的質(zhì)粒中的sgRNA基于碧云天研發(fā)的CRISPR/Cas9 sgRNA快速篩選和驗(yàn)證體系獲得，sgRNA的有效性已經(jīng)通過T7EI法驗(yàn)證。
AANAT基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | AANAT | 15 | BC069434 | NM_001088 |
About the gene | |
Official Symbol | AANAT |
Previous Symbol | - |
Official Full Name | aralkylamine N-acetyltransferase |
Synonyms | SNAT |
Location | 17q25.1 |
Gene Type | protein-coding gene |
Uniprot ID | Q16613 |
Pathway/Library | cAMP & Ca Signaling Pathway Related Genes Library |
Gene Summary | Arylalkylamine N-acetyltransferase belongs to the superfamily of acetyltransferases. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle. |
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
| L00102 | AANAT Knockout Lentivirus | 108 TU |
| — | 說明書 | 1份 |
-80℃保存，至少一年有效。
本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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