TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?-標準-資訊-生物在線

TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

作者：Agrisera AB 2020-10-21T00:00 (訪問量:4828)

?TBS-T or PBS-T, does it matter which buffer is used for antibody incubations??

Oftentimes TBS-T is a buffer of choice applied in many Western blot protocols for all antibody incubations.?

However, as you can see in this example, changing to PBS-T has also changed a pattern of detected bands. Analysed samples (Arabidopsis thaliana), protein load/well, primary, secondary antibodies and detection reagent were kept the same.?

Antibody incubation buffer has been changed from?TBS-T?(left pannel) to?PBS-T(right panel).
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 ? Western blot with anti-catalase antibodies


Western blot protocol with all details for this experiment can be found?here.?

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