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| 中文名稱 | 蛋白質磷酸酶2A （alpha + beta）抗體 |
| 別????名 | Medium tumour antigen associated 61kDa protein; MGC786; PP2A beta; PP2A PR65 alpha isoform; PP2A-beta; PP2AB_HUMAN; PP2Abeta; PP2CB; Ppp2cb; Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; Protein phosphatase 2 catalytic subunit beta isozyme; Protein phosphatase 2 regulatory subunit A alpha isoform; Protein phosphatase 2, catalytic subunit, beta isoform; Protein phosphatase 2A catalytic subunit beta isoform; Protein phosphatase type 2A catalytic subunit; Serine threonine protein phosphatase 65 kDa; Serine/threonine protein phosphatase 2A catalytic subunit beta; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform.?? |
| 研究領域 | 細胞生物??信號轉導??細胞周期蛋白??激酶和磷酸酶?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,? (predicted: Rat,?Chicken,?Pig,?Cow,?Horse,?Rabbit,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 36kDa |
| 細胞定位 | 細胞核?細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human PP2A beta/PPP2CB:21-120/309? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]. Function: PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Subunit: Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD (By similarity). PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1. May indirectly interact with SGOL1, most probably through regulatory B56 subunits. Subcellular Location: Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Post-translational modifications: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro). Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily. SWISS: P62714 Gene ID: 5515 Database links: Entrez Gene: 5515?Human Entrez Gene: 5516?Human Entrez Gene: 19052?Mouse Entrez Gene: 19053?Mouse Entrez Gene: 24672?Rat Entrez Gene: 24673?Rat Omim: 176915?Human Omim: 176916?Human SwissProt: P62714?Human SwissProt: P67775?Human SwissProt: P62715?Mouse SwissProt: P63330?Mouse SwissProt: P62716?Rat SwissProt: P63331?Rat Unigene: 105818?Human Unigene: 491440?Human Unigene: 260288?Mouse Unigene: 288765?Mouse Unigene: 1271?Rat Unigene: 977?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-PP2A beta (bs-6706R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 40 kD  Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-PP2A beta (bs-6706R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 40 kD  Sample:U87MG(Human) Cell Lysate at 30 ug Primary: Anti- PP2A alpha + beta (bs-6706R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD  Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP2A beta) Polyclonal Antibody, Unconjugated (bs-6706R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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