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| 中文名稱 | CD3E抗體 |
| 別????名 | CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon (CD3 TCR complex); CD3e molecule, epsilon (CD3-TCR complex); CD3E_HUMAN; CD3E_MOUSE; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE.?? |
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| 研究領域 | 腫瘤??細胞生物??免疫學??淋巴細胞??t-淋巴細胞?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,? (predicted: Rat,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?Flow-Cyt=2ug/Test? not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 20kDa |
| 細胞定位 | 細胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from mouse CD3 epsilon:23-120/189? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. Function: The CD3 complex mediates signal transduction. Subunit: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain. SWISS: P22646 Gene ID: 12501 Database links: Entrez Gene: 916?Human Entrez Gene: 12501?Mouse Omim: 186830?Human SwissProt: P07766?Human SwissProt: P22646?Mouse Unigene: 3003?Human Unigene: 210361?Mouse ? Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-CD3E (bs-4815R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Blank control:Mouse bone marrow.Primary Antibody (green line): Rabbit Anti-CD3E antibody (bs-4815R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: mouse bloodIsotype Control Antibody: Rabbit IgG -PE-CY5; Primary Antibody Dilution: 1 μl in 100 μL1X PBS containing 0.5% BSA.  Blank control (blue line): Mouse spleen cells(fixed with 70% ice-cold methanol overnight at 4℃).Primary Antibody (green line): Rabbit Anti-CD3E/PE-CY7 Conjugated antibody (bs-4815R-PE-CY7),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 . |
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