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| 中文名稱 | 精子頂體前體蛋白抗體 |
| 別????名 | ACR; Acrosin precursor; ACRS;Preproacrosin; Proacrosin; ACRO_HUMAN.?? |
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| 研究領域 | 細胞生物??免疫學??泛素?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,?Rat,? (predicted: Dog,?Pig,?Cow,?Horse,?Rabbit,?Sheep,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=1ug/Test?IF=1:50-200?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 33/44kDa |
| 細胞定位 | 細胞核?細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Acrosin heavy chain:201-300/421? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. Function: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Subunit: Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms heterodimer with SERPINA5. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: P10323 Gene ID: 49 Database links: Entrez Gene: 49?Human Omim: 102480?Human SwissProt: P10323?Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Epididymis (Mouse) Lysate at 40 ug Primary: Anti-Acrosin (bs-5151R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33/44kD Observed band size: 33 kD  Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Acrosin) Polyclonal Antibody, Unconjugated (bs-5151R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Acrosin) Polyclonal Antibody, Unconjugated (bs-5151R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining Blank control: HepG2.Primary Antibody (green line): Rabbit Anti-Acrosin antibody (bs-5151R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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