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| 中文名稱 | G氨基丁酸受體3抗體 |
| 別????名 | GABA A Receptor beta 3; ECA5; GABA alpha receptor beta-2 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta 3 subunit; GABAA receptor subunit beta 3; GABR B3; Gabrb3; Gamma aminobutyric acid (GABA) A receptor beta 3; Gamma aminobutyric acid receptor subunit beta 3; Gamma-aminobutyric acid receptor subunit beta-3; GBRB3_HUMAN; MGC9051.?? |
| 研究領域 | 細胞生物??神經生物學??信號轉導??通道蛋白??細胞膜受體?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,?Rat,? (predicted: Human,?Chicken,?Pig,?Cow,?Horse,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=1μg/Test?ICC=1:100-500?IF=1:50-200?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 52kDa |
| 細胞定位 | 細胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human GABR B3/GABA A Receptor beta 3:31-130/473? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013] Function: Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. Subunit: Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Can form functional homopentamers (in vitro). Interacts with UBQLN1. May interact with KIF21B. Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 . Interacts with LHFPL4. Subcellular Location: Plasma membrane Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. SWISS: P28472 Gene ID: 2562 Database links: Entrez Gene: 2562?Human Entrez Gene: 14402?Mouse Entrez Gene: 24922?Rat Omim: 137192?Human SwissProt: P28472?Human SwissProt: P63080?Mouse SwissProt: P63079?Rat Unigene: 302352?Human Unigene: 727263?Human Unigene: 8004?Mouse Unigene: 233948?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Cerebrum (Rat) Lysate at 40 ug Primary: Anti-GABR B3 (bs-8544R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-GABR B3 (bs-8544R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-GABR B3 (bs-8544R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 48 kD  Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)).Primary Antibody:Rabbit Anti- GABR B3 antibody(bs-8544R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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