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| 中文名稱 | S100P結合蛋白抗體 |
| 別????名 | S100P-binding protein; S100P-binding protein Riken;y S100PBP; S100PBPR; S1PBP_HUMAN.?? |
| 研究領域 | 腫瘤??信號轉導??轉錄調節因子??結合蛋白??腫瘤細胞生物標志物?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,? (predicted: Rat,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=2ug/Test?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 46kDa |
| 細胞定位 | 細胞核? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human S100PBP:331-408/408? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | S100PBP was originally cloned from a pancreatic epithelioid carcinoma library and encodes a predicted 408 amino acid protein. RT-PCR detected S100PBP expression in brain, breast, spleen, and lung, but not in pancreas and liver. GFP-tagged S100PBP localized to nuclei of transfected HeLa cells. Subunit: Interacts with S100P. Subcellular Location: Nucleus. Note=Colocalizes with S100P in the nucleus. Tissue Specificity: Expressed in brain, spleen, and lung. Not detected in pancreas or liver. In pancreas, expressed predominantly in islet cells and to a lesser extent in acinar cells, but not expressed in ductal cells. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Detected in pancreatic ductal adenocarcinoma cells (at protein level). Not detected in non-neoplastic ductal epithelium (at protein level). SWISS: Q96BU1 Gene ID: 64766 Database links: Entrez Gene: 64766?Human Omim: 611889?Human SwissProt: Q96BU1?Human Unigee: 440880?Human ? Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Molt-4 (Human) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- S100P binding protein (bs-6577R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD  Sample:Spleen (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti- S100P binding protein (bs-6577R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD  Blank control:Mouse spleen.Primary Antibody (green line): Rabbit Anti-S100P binding protein antibody (bs-6577R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488R Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-S100P binding protein antibody (bs-6577R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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