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| 中文名稱 | 14-3-3 protein ζ/δ抗體 |
| 別????名 | 14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14-3-3 protein zeta/delta; 14-3-3zeta; 14-3-3 delta; 14-3-3 zeta; 14-3-3delta; 1433Z_HUMAN; KCIP 1; KCIP-1; KCIP1; MGC111427; MGC126532; MGC13815; Phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide; YWHAZ.?? |
| 研究領域 | 腫瘤??細胞生物??神經生物學??信號轉導??細胞凋亡??轉錄調節因子?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,?Rat,? (predicted: Chicken,?Dog,?Cow,?Horse,?Rabbit,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?Flow-Cyt=1ug/Test? not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 27kDa |
| 細胞定位 | 細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta:51-150/245? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Subunit: Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2. Subcellular Location: Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes. Post-translational modifications: The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. Similarity: Belongs to the 14-3-3 family. SWISS: P63104 Gene ID: 7534 Database links: Entrez Gene: 7529?Human Entrez Gene: 7534?Human Entrez Gene: 22631?Mouse Entrez Gene: 54401?Mouse Entrez Gene: 25578?Rat Entrez Gene: 56011?Rat Omim: 601288?Human Omim: 601289?Human SwissProt: P29358?Cow SwissProt: P31946?Human SwissProt: P63104?Human SwissProt: P35215?Mouse SwissProt: Q9CQV8?Mouse SwissProt: P35213?Rat SwissProt: P63102?Rat SwissProt: P29361?Sheep Unigene: 492407?Human Unigene: 643544?Human Unigene: 1292?Rat Unigene: 8653?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-14-3-3 zeta+delta (bs-6790R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD  Sample:Cerebrum (Rat) Lysate at 40 ug Primary: Anti-14-3-3 zeta+delta (bs-6790R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD  Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (bs-6790R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<, Dilution: 1μg /test.  Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (bs-6790R) ; Dilution: 1μg /10^6 cells; Isotype Control Antibody (grenn line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test. |
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