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| 中文名稱 | 血小板源性生長因子A抗體 |
| 別????名 | Platelet–dirived growth factor–1; PDGFA; PDGF AA; PDGF-AA; PDGF A chain; PDGF subunit A; PDGF-1; PDGF1; PDGFA; PDGFA_HUMAN; Platelet derived growth factor A chain precursor; Platelet derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A.?? |
| 研究領域 | 腫瘤??免疫學??神經生物學??生長因子和激素?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,? (predicted: Rat,?Rabbit,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=0.2μg/Test?ICC=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 14kDa |
| 細胞定位 | 分泌型蛋白? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human PDGF-A:102-213/213? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. The long form contains a basic insert which acts as a cell retention signal. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. It belongs to the PDGF/VEGF growth factor family. Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Subunit: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4. Subcellular Location: Secreted. Note=Released by platelets upon wounding. Tissue Specificity: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Similarity: Belongs to the PDGF/VEGF growth factor family. SWISS: P01127 Gene ID: 5154 Database links: Entrez Gene: 5154?Human Entrez Gene: 5155?Human Entrez Gene: 18590?Mouse Entrez Gene: 18591?Mouse Entrez Gene: 24628?Rat Entrez Gene: 25266?Rat Omim: 173430?Human Omim: 190040?Human SwissProt: P01127?Human SwissProt: P04085?Human SwissProt: P20033?Mouse SwissProt: P31240?Mouse SwissProt: P28576?Rat SwissProt: Q05028?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PDGF-A（platelet-dirived growth factor-A）是一種堿性蛋白，pdgf促進細胞分裂、增值同時，可誘導c-fos、c-myc等蛋白表達增強。 主要作用：參與組織和血管修復、促進平滑肌細胞、成纖維細胞迅速增值。分子量為28-35kDa，分為兩個亞基（A、B鏈）通過二硫鍵結合而成的兩條多肽鏈的異二聚體。最新研究發現了PDGF家族兩種新的基因PDGF-C、PDGF-D。 |
| 產品圖片 |  Sample:Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Uterus (Mouse) Lysate at 40 ug Lane 3: Siha (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-PDGF-A (bs-10073R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDGF-A) Polyclonal Antibody, Unconjugated (bs-10073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDGF-A) Polyclonal Antibody, Unconjugated (bs-10073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).Primary Antibody (green line): Rabbit Anti-PDGF-A antibody (bs-10073R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1μg /test. |
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