免疫球蛋白樣結構域受體1抗體-軟件手冊-資訊-生物在線

免疫球蛋白樣結構域受體1抗體

作者:上海雅吉生物科技有限公司 2020-11-09T00:00 (訪問量:2839)

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中文名稱 免疫球蛋白樣結構域受體1抗體
別????名 ILDR1alpha; ILDR1beta; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1; Immunoglobulin like domain containing receptor 1 beta; MGC50831; ILDR1_HUMAN.??
研究領域 細胞生物??免疫學??
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,?Mouse,?Rat,? (predicted: Pig,?Cow,?Horse,?Rabbit,?Sheep,?)
產品應用 WB=1:500-2000?ELISA=1:500-1000?Flow-Cyt=3ug/Test?
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量 60kDa
細胞定位 細胞漿?細胞膜?
性????狀 Liquid
濃????度 1mg/ml
免?疫?原 KLH conjugated synthetic peptide derived from human ILDR1:101-200/546?
亞????型 IgG
純化方法 affinity purified by Protein A
儲?存?液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 ILDR1 is a putative membrane receptor. It belongs to the immunoglobulin superfamily, LISCH7 family. It is mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver.

Function:
Putative membrane receptor.

Subunit:
Homooligomer.

Subcellular Location:
Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol).

Tissue Specificity:
Mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver.

DISEASE:
Defects in ILDR1 are the cause of deafness autosomal recessive type 42 (DFNB42) [MIM:609646]; also called non-syndromic sensorineural deafness autosomal recessive type 42. DFNB42 is a prelingual, non-progressive form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Similarity:
Belongs to the immunoglobulin superfamily. LISCH7 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q86SU0

Gene ID:
286676

Database links:

Entrez Gene: 286676?Human

Entrez Gene: 106347?Mouse

Omim: 609739?Human

SwissProt: Q86SU0?Human

SwissProt: Q8CBR1?Mouse

Unigene: 98484?Human

Unigene: 17807?Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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產品圖片 Sample:
Prostate(Rat) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
Pancreas(Mouse) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (bs-11013R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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