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| 中文名稱 | 鈣蛋白酶抑制蛋白抗體 |
| 別????名 | BS 17; Calpain inhibitor; Calpastatin; CAST; Heart type calpastatin; ICAL_HUMAN; MGC9402; Sperm BS 17 component; Sperm BS-17 component.?? |
| 研究領域 | 細胞生物??神經生物學??細胞類型標志物?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,? (predicted: Rat,?Cow,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 70kDa |
| 細胞定位 | 細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Calpastatin:431-530/708? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases that mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Cal-pain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Function: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the protease inhibitor I27 (calpastatin) family. SWISS: P20810 Gene ID: 831 Database links: Entrez Gene: 831?Human Entrez Gene: 12380?Mouse Entrez Gene: 25403?Rat Omim: 114090?Human SwissProt: P20810?Human SwissProt: P51125?Mouse SwissProt: P27321?Rat Unigene: 436186?Human Unigene: 29163?Mouse Unigene: 441995?Mouse Unigene: 17481?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample: Lung (Mouse) Lysate at 40 ugPrimary: Anti-Calpastatin (bs-6701R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 65 kD  Sample: Raji Cell (Human) Lysate at 40 ugPrimary: Anti-Calpastatin (bs-6701R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 65 kD  Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpastatin) Polyclonal Antibody, Unconjugated (bs-6701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpastatin) Polyclonal Antibody, Unconjugated (bs-6701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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