當對小鼠樣本進行 IHC 實驗時，CST 更推薦您使用兔單克隆抗體而不是鼠單克隆抗體，為什么，這么做有什么好處？3 步讓您了解原因！
使用小鼠單克隆抗體會造成小鼠組織結果無法解讀
如經適當驗證，小鼠單克隆抗體會特異性地對目的靶標染色，但數據解讀很可能因使用抗小鼠 IgG 檢測試劑（二抗）同時能檢測內源性小鼠 IgG 而受到影響。如圖1，依次使用一種 α-平滑肌肌動蛋白（α-SMA）小鼠單克隆抗體和一種抗小鼠 IgG 檢測試劑對石蠟包埋的小鼠肺細胞進行免疫組織化學 (IHC) 分析，結果顯示出預期的特異性染色，但由于存在抗小鼠 IgG 二抗，還顯示出背景染色過度。避免小鼠 IgG 背景的唯一辦法是在你的實驗步驟中添加費力的步驟，或換用一種非嚙齒類抗體，例如聯合使用兔單克隆抗體和抗兔 IgG 的二抗。
圖1 ，注：小鼠 IgG 二抗導致背景染色過度。
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抗小鼠 IgG 二抗會造成小鼠抗小鼠背景染色
使用小鼠一抗需要抗小鼠 IgG 二抗或檢測試劑，這同時會對一抗和小鼠組織樣品的 IgG 蛋白進行染色。為了讓您直觀地觀察，圖2展示的是在沒有一抗的情況下，使用 SignalStain? Boost IHC Detection Reagent (HRP, Mouse) #8125 對石蠟包埋的小鼠肺細胞進行 IHC 分析。
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兔單克隆抗體無需額外的實驗步驟即可產生可解讀的小鼠組織結果
使用在小鼠組織中經 IHC 驗證的兔單克隆一抗可克服小鼠抗小鼠染色，并發現明確且可解讀的結果。圖3 所示的是使用 α-Smooth Muscle Actin (D4K9N) XP? Rabbit mAb #19245 對石蠟包埋的小鼠肺細胞進行的 IHC 分析。
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產品名稱 |
貨號 |
應用 |
反應性 |
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細胞生物學 |
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Bax (D3R2M) Rabbit mAb (Rodent Preferred) |
WB、IP、IHC-P |
M、R |
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N-Cadherin (D4R1H) XP? Rabbit mAb |
WB、IP、IHC-P、IF-IC |
H、M |
|
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CD44 (E7K2Y) XP? Rabbit mAb |
WB、IHC-P |
H、M、R |
|
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EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) |
WB、IP、IHC-P、 IF-F、IF-IC、F |
M |
|
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EpCAM (E6V8Y) XP? Rabbit mAb (Mouse Preferred) |
WB、IP、IHC-P |
H、M、R |
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HER3/ErbB3 (D22C5) XP? Rabbit mAb |
WB、IP、IHC-P、 IF-IC、F |
H、M、(R) |
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Integrin ?3 (D7X3P) XP? Rabbit mAb |
WB、IHC-P |
H、M |
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Keratin 5 (E2T4B) XP? Rabbit mAb (IHC Preferred) |
IHC-P |
H、M |
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Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) |
IHC-P |
M |
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Olfm4 (D6Y5A) XP? Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、IF-F |
M |
|
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Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、 IF-IC、F |
M、R |
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PCNA (D3H8P) XP? Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk |
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Perforin (E3W4I) Rabbit mAb |
WB、IHC-P |
M |
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α-Smooth Muscle Actin (D4K9N) XP? Rabbit mAb |
WB、IP、IHC-P、IF-F |
H、M、R |
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Vinculin (E1E9V) XP? Rabbit mAb |
WB、IHC-P、F |
H、M、R、Mk |
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發育生物學 |
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Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP? Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R、Mk、 Z、Dg |
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HES1 (D6P2U) Rabbit mAb |
WB、IP、IHC-P |
H、M、R、Mk |
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SMAD4 (D3R4N) XP? Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
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Sox9 (D8G8H) Rabbit mAb |
WB、IHC-P、IF-IC、F |
H、M、(M、R) |
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YAP (D8H1X) XP? Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Hm、 Mk、(B、Hr) |
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表觀遺傳學 |
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ARID1A/BAF250A (D2A8U) Rabbit mAb |
WB、IHC-P、ChIP |
H、M、R、Mk |
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EED (E4L6E) XP? Rabbit mAb |
WB、IP、IHC-P、IF-IC、 F、ChIP |
H、M、R、Mk |
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Glucocorticoid Receptor (D6H2L) XP? Rabbit mAb |
WB、IP、HC-P、IF-IC、 F、ChIP、ChIP-seq |
H、M、R、Mk |
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代謝 |
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Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb |
WB、IP、IHC-P |
H、M、R、C、Z、 B、Pg) |
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Arginase-1 (D4E3MTM ) XP? Rabbit mAb |
WB、IHC-P、IF-F、 IF-IC、F |
H、M、R |
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COX IV (D6I4K) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P |
M、R |
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SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、IF-IC |
M、R |
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神經科學 |
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Adora2a/Adenosine Receptor A2a (E5I8X) XP? Rabbit mAb (Rodent Specific) |
IHC-P、IF-F |
M、R |
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GFAP (E4L7M) XP? Rabbit mAb |
WB、IHC-P、IF-F |
H、M、R |
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Myelin Basic Protein (D8X4Q) XP? Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
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NCAM1 (CD56) (E7X9M) XP? Rabbit mAb |
WB、IP、IHC-P、IF-F、 IF-IC、F |
H、M、R |
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NeuN (D4G4O) XP? Rabbit mAb |
WB、IHC-P、IF-F |
H、M、R |
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Tau (D1M9X) XP? Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
H、M、R |
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免疫學 |
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Btk (D3H5) Rabbit mAb |
WB、IP、IHC-P、F |
H、M、(R、Hm、B、Dg、Pg、Hr) |
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CD3ε (D4V8L) Rabbit mAb |
WB、IHC-P |
M |
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CD4 (D7D2Z) Rabbit mAb |
WB、IHC-P |
M、R |
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CD8α (D4W2Z) XP? Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
|
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CD11c (D1V9Y) Rabbit mAb |
WB、IHC-P、IF-F、IF-IC |
M |
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CD19 (D4V4B) XP? Rabbit mAb |
WB、IP、IHC-P |
H、M、(B) |
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CD31 (PECAM-1) (D8V9E) XP? Rabbit mAb |
WB、IHC-P |
M |
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CD39/NTPDase 1 (E2X6B) XP? Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
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CD40 (E2Z7J) Rabbit mAb |
WB、IP、IHC-P |
M |
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CD45 (D3F8Q) Rabbit mAb |
P、IHC-P、IF-F、IF-IC |
M |
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F4/80 (D2S9R) XP? Rabbit mAb |
WB、IP、IHC-P |
M |
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Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、F |
M |
|
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FoxP3 (D6O8R) Rabbit mAb |
IHC-P、IF-F、F |
M |
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FcγRIIB (D8F9C) XP? Rabbit mAb（Mouse Specific) |
WB、IP、IHC-P、F |
M |
|
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NT5E/CD73 (D7F9A) Rabbit mAb |
WB、IHC-P |
H、M、R |
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PD-1 (D7D5W) XP? Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P、IF-F、IF-IC、F |
M、(R、Hm) |
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PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) |
IHC-P |
M |
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PD-L2 (D6L5A) Rabbit mAb |
IHC-P |
M |
|
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S100A9 (D3U8M) Rabbit mAb (Rodent Specific) |
WB、IP、IHC-P、 IF-F、F |
M、R |
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STING (D2P2F) Rabbit mAb |
WB、IP、IHC-P |
H、M |
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TIM-3 (D3M9R) XP? Rabbit mAb (Mouse Specific) |
WB、IP、IHC-P |
M |
|
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VCAM-1 (D2T4N) Rabbit mAb (Mouse Specific) |
WB、IHC-P |
M |
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