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| 中文名稱 | 沉默調節樣蛋白SirT7抗體 |
| 別????名 | NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7.? |
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| 研究領域 | 腫瘤??心血管??免疫學??染色質和核信號??信號轉導??細胞凋亡??轉錄調節因子??激酶和磷酸酶??結合蛋白??表觀遺傳學?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,? (predicted: Human,?Rat,?Chicken,?Dog,?Pig,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=1μg/Test?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 45kDa |
| 細胞定位 | 細胞核?細胞漿? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human SIRT7:231-330/400? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Function: NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. Subunit: Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B. Subcellular Location: Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. Post-translational modifications: Phosphorylated during mitosis (Probable). Similarity: Belongs to the sirtuin family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain. SWISS: Q9NRC8 Gene ID: 51547 Database links: Entrez Gene: 505662?Cow Entrez Gene: 51547?Human Entrez Gene: 209011?Mouse Omim: 606212?Human SwissProt: Q0P595?Cow SwissProt: Q9NRC8?Human SwissProt: Q8BKJ9?Mouse Unigene: 514636?Human Unigene: 292957?Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Small intestine (Mouse)Lysate at 40 ugPrimary: Anti-SIRT7(bs-5973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD  Sample:Lymph node (Mouse)Lysate at 40 ugPrimary: Anti-SIRT7(bs-5973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD  Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: mouse splenocytes(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |