| Can tubulin be used as a?loading control in metal stress? To check this??Anti-tubulin alpha chain antibodies?were used on extracts from: Nicotiana tabacum?plants were?treated with?silver nanoparticles (AgNPs) with 2 different coatings. Tubulin alpha protein content marked by a blue arrow in the image to the right, was influenced by silver nanoparticle treatment.?Similar results were obtained for?tubulin gamma chain.? Therefore, it can be concluded that: Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading control under these conditions. | ? |  All experimental details can be found?here. Courtesy of MSc, Karla Ko?pi?, University of Zagreb, Faculty of Science Department of Biology, Croatia |
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Tubulin alpha and gamma are not suitable as a loading control for metal stress
作者：Agrisera AB 2021-04-12T00:00 (訪問量:6474)
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