DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures. The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the matrix in each well of the spin-plate, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.
HIGHLIGHTS:
Quick, high-throughput (96-well spin plate) purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood, saliva, swab, fecal, etc.
Omits the use of organic denaturants and proteases.
High-quality viral RNA is ready for RT-PCR, sequencing, etc.