人活性Apg3全長蛋白 信號轉(zhuǎn)導(dǎo)蛋白
產(chǎn)品名稱： 人活性Apg3全長蛋白 信號轉(zhuǎn)導(dǎo)蛋白
英文名稱： 2610016C12Rik Apg 3 APG3 APG3 autophagy 3 like APG3 like APG3, S. cerevisiae, homolog of APG3-like APG3L Apg3p ATG 3 ATG3 ATG3 autophagy rel
產(chǎn)品編號： ab188738
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 英國
品牌商標(biāo)： abcam
更新時(shí)間： null
使用范圍： null
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : 1484332550@qq.com
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- 性質(zhì)Recombinant
- 來源E. coli
- 氨基酸序列
- AccessionQ9NT62
- 種屬Human
- 序列MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHC PTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE DDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDED EGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTY DLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENH PHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAV IPTIEYDYTRHFTM
- 分子量37 kDa including tags
- 氨基酸1 to 314
- 標(biāo)簽His tag N-Terminus
技術(shù)指標(biāo)
Our?Abpromise guarantee?covers the use of?ab188738?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性
Typical enzyme concentration to support conjugation?in vitro?is 100 nM-1 μM depending on conditions.
- 應(yīng)用
SDS-PAGE
Functional Studies
- 純度>95% by SDS-PAGE .
- 形式Liquid
- ?
制備和貯存
- 穩(wěn)定性和存儲(chǔ)
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 10% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常規(guī)信息
-
別名
- 2610016C12Rik
- Apg 3
- APG3
see all - 功能E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
- 組織特異性Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
- 序列相似性Belongs to the ATG3 family.
- 翻譯后修飾Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
- 細(xì)胞定位Cytoplasm.
- ?