Anti-KIT (D820E) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-KIT (D820E) Mouse Monoclonal Antibody

Anti-KIT (D820E) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-KIT (D820E) Mouse Monoclonal Antibody

英文名稱： Anti-KIT (D820E) Mouse Monoclonal Antibody

產品編號： 26274

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC

武漢紐斯特生物技術有限公司
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KIT (D820E) [26274]
[100ul]		 ?

Catalog Number:??26274
Gene Symbol:??C-Kit; CD117; KIT; PBT; SCFR
Description: ??Anti-KIT (D820E) Mouse Monoclonal Antibody
Background: ??Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.
Immunogen: ??A synthetic peptide from the internal region of KIT which includes the mutation of D820E, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:500-1:1000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes D820E mutant, but not wild-type KIT of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant KIT (D820E) and wild-type proteins. Purified His-tagged KIT (D820E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-KIT (D820E) monoclonal antibody (Cat. #26274).
Immunofluorescence:??
Immunofluorescence of cells expressing KIT proteins with anti-KIT (D820E) antibody. HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT (D820E) plasmid (right column), then fixed and stained with anti- KIT (D820E) monoclonal antibody (Cat. #26274).