DGKG polyclonal antibody
產(chǎn)品名稱： DGKG polyclonal antibody
英文名稱： DGKG polyclonal antibody
產(chǎn)品編號： PAB3342
產(chǎn)品價格： null
產(chǎn)品產(chǎn)地： 臺灣
品牌商標(biāo)： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯(lián)系人 :
- 地址 : 臺灣臺北市內(nèi)湖區(qū)洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區(qū)域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DGKG.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DGKG.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells.
Yamada K, Sakane F, Imai S, Tsushima S, Murakami T, Kanoh H.Biochem Biophys Res Commun. 2003 May 23;305(1):101-7.
- 2.
- Mapping and genomic characterization of the gene encoding diacylglycerol kinase gamma (DAGK3): assessment of its role in dominant optic atrophy (OPA1).
Stohr H, Klein J, Gehrig A, Koehler MR, Jurklies B, Kellner U, Leo-Kottler B, Schmid M, Weber BH.Hum Genet. 1999 Jan;104(1):99-105.
- 3.
- Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells.
Kai M, Sakane F, Imai S, Wada I, Kanoh H.J Biol Chem. 1994 Jul 15;269(28):18492-8.
- Applications
- Western Blot (Tissue lysate)
- The DGKG polyclonal antibody (Cat # PAB3342) is used in Western blot to detect DGKG in mouse small intestine tissue lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKG polyclonal antibody (Cat # PAB3342) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 1608
- Protein Accession#:
- P49619
- Gene Name:
- DGKG
- Gene Alias:
- DAGK3,DGK-GAMMA,MGC104993,MGC133330
- Gene Description:
- diacylglycerol kinase, gamma 90kDa
- Omim ID:
- 601854
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- DAG kinase gamma,diacylglycerol kinase gamma,diacylglyerol kinase gamma,diglyceride kinase gamma