半胱胺酸蛋白酶蛋白-12抗體說明書
產(chǎn)品名稱: 半胱胺酸蛋白酶蛋白-12抗體說明書
英文名稱: 電詢
產(chǎn)品編號(hào): YM-H15831-X
產(chǎn)品價(jià)格: null
產(chǎn)品產(chǎn)地: 上海
品牌商標(biāo): 上海遠(yuǎn)慕
更新時(shí)間: null
使用范圍: 科研實(shí)驗(yàn)
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- 郵編 : 201200
- 所在區(qū)域 : 上海
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產(chǎn)品名稱:半胱胺酸蛋白酶蛋白-12抗體說明書
產(chǎn)品貨號(hào):YM-H15831-X
濃 度: 1mg/1ml
貯 存: 貯存于-20℃.
亞 型: IgG
性 狀:Lyophilized or Liquid
產(chǎn)品類型: 一抗 多抗體
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
半胱胺酸蛋白酶蛋白-12抗體說明書產(chǎn)品WB實(shí)驗(yàn)中的對照系統(tǒng):
he chromogenic reaction (Note: two anti generally have two kinds ofcommonly used marker enzyme, HRP and AP, chromogenic substrate kit andits corresponding to DAB and BCIP/NBT, the general use ofchemiluminescence lysate.) Westernbloting is the first to transfer afterelectrophoresis separation of proteins from the gel onto a NC membrane,there are generally two ways: capillary blotting and electrophoreticblotting. Westernbloting is a common method used to detect the protein,through the immune reaction of antigen antibody can be quantitativelymeasured the relative molecular weight of antibody, and the relativeabundance of known antigen or antibody and the relation between. Atpresent, it is applied in many fields, detection of infectious diseasesand autoimmune diseases, allergies, etc. in clinic, in antibodyproduction field it with ELISA, ICC, IHC, IFA and other immunetechnology has become the main means of engaging a screening ofmonoclonal antibody production.
WB對照系統(tǒng)
陽性對照:最好有標(biāo)準(zhǔn)品,或陽性血清、陽性上清。
陰性對照:測血時(shí)用相應(yīng)小鼠未免疫血清,測培養(yǎng)上清,用無克隆培養(yǎng)上清。
空白對照:不加一抗,用1%BSA代替。
無關(guān)對照(替代對照):用無關(guān)項(xiàng)目一抗,或無關(guān)項(xiàng)目的抗體。
半胱胺酸蛋白酶蛋白-12抗體說明書產(chǎn)品名稱:
半胱胺酸蛋白酶蛋白-12抗體說明書
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