Size??100ul
Cat code??220030
Species Cross-reactivity??H
Key Application??WB, ICC
Host??Mouse
Clone Number??5D11-B8-H9
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG1
Molecular Weight??25kDa
Sensitivity??The antibody detects endogenous level of EIF4E protein.
Immunogen??Purified recombinant fragment of human EIF4E expressed in E. Coli.
UniProt Number??P06730
GeneBank ID??NM_001968.3
Subcellular location??Cytoplasm ? P-body
Formulation??Ascitic fluid containing 0.03% sodium azide.
Altername??AUTS19; CBP; CBP; eIF 4E; eIF 4F 25 kDa subunit; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4EL1; EIF4F; EIF4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; MGC111573; mRNA cap binding protein; mRNA cap-binding protein;
Gene_symbol??CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Summary??The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5′ end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniPort summary  Function??Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Application Images :??
Western blot detection of EIF4E antibody in 293, K562 and Hela cell lysates using EIF4E antibody (dilution 1:1000).Predicted band size:25 KDa.Observed band size:28KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-EIF4E antibody (dilution 1:100).