The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of sheared DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR. 

Advantages:
Enjoy flexible input amounts from 250 ng to 1,000 ng of sheared DNA.
Get more of what you need with reliably high library yields.
Prepare PCR-free libraries with a broad range of sample types.
Save time with streamlined workflows, reduced hands-on time, and automation compatibility.
Use with the NEBNext Multiplex Oligos for Illumina (Unique Dual Index. UMI Adaptors DNA Set 1) or other Illumina-compatible index adaptors with a 3’ single T overhang.