?中文名稱?血管內皮生長因子受體1重組兔單克隆抗體?
別? ? 名 VEGF-R1; VEGFR-1; VEGF Receptor 1; FLT-1; vascular permeability factor receptor; vascular endothelial growth factor receptor; vascular endothelial growth factor receptor-1; fms-related tyrosine kinase 1; vascular endothelial growth factor/vascular permeability factor receptor; AI323757; FLT; FLT1; sFlt1; VGFR1_HUMAN; VGFR1_MOUSE.? ?
研究領域 腫瘤? 細胞生物? 免疫學? 發育生物學? 神經生物學? 生長因子和激素? 激酶和磷酸酶? 細胞膜受體? 細胞表面分子? ?
抗體來源 Rabbit?
克隆類型 Monoclonal?
克 隆 號 3A6?
交叉反應 Human, Mouse,? (predicted: Rat, )?
產品應用 WB=1:1000-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.?
分 子 量 147kDa?
細胞定位 細胞漿 細胞膜 分泌型蛋白??
性? ? 狀 Liquid?
濃? ? 度 1mg/ml?
免 疫 原 KLH conjugated synthetic peptide derived from human VEGFR1:??
亞? ? 型 IgG?
純化方法 affinity purified by Protein A?
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.?
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.?
PubMed PubMed?
產品介紹 VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.
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Function:
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiog
產品圖片
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 4: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-VEGFR1 (bsm-52338R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 180 kD
Observed band size: 190 kD
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFR1) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.