Marcks (phospho S152/S156) polyclonal antibody
產品名稱： Marcks (phospho S152/S156) polyclonal antibody
英文名稱： Marcks (phospho S152/S156) polyclonal antibody
產品編號： PAB9642
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
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- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Marcks.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S152/S156 of rat Marcks.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 87
- Reactivity:
- Bovine, Chicken, Clawed frog, Human, Mouse, Rat, Zebra fish
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163.
Palmer RH, Schonwasser DC, Rahman D, Pappin DJ, Herget T, Parker PJ.FEBS Lett. 1996 Jan 15;378(3):281-5.
- 2.
- Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein.
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ.J Biol Chem. 1994 Mar 25;269(12):9361-7.
- 3.
- Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain.
Ouimet CC, Wang JK, Walaas SI, Albert KA, Greengard P.J Neurosci. 1990 May;10(5):1683-98.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat brain lysate showing specific immunolabeling of the ~87k Marcks protein phosphorylated at Serr152,156 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Marcks Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.