DH5α 化學感受態細胞
產品名稱： DH5α 化學感受態細胞
英文名稱： DH5α Chemically Competent Cell DH5α
產品編號： 11802ES
產品價格： 詢價
產品產地： 翌圣生物
品牌商標： Yeasen
更新時間： 2025-06-19T09:54:17
使用范圍： null
- 聯系人 : 李自轉
- 地址 : 上海市浦東新區天雄路166弄一號樓三層南單元
- 郵編 : 200030
- 所在區域 : 上海
- 電話 : 139****5640 點擊查看
- 傳真 : 點擊查看
- 郵箱 : lizizhuan@yeasen.com
- 二維碼 : 點擊查看
本品是采用DH5α菌株經特殊工藝處理得到的感受態細胞，可用于DNA的化學轉化，廣泛適用于高效的DNA克隆和質粒擴增，能保證高拷貝質粒的穩定遺傳，適用于藍白斑篩選。DH5α細胞重組酶recA1和核酸內切酶endA1基因缺失突變使其能保證克隆DNA的穩定性。使用pUC19質粒檢測，轉化效率約達108 cfu/μg DNA。
DH5α細胞基因型：F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk-,mk+) supE44λ- thi -1 gyrA96 relA1phoA
- 轉化效率高
干冰運輸；-80℃保存，有效期6個月。請勿將本品置于-20℃或者液氮中保存。
Q：最大可以轉化多大的質粒？
A：轉化質粒的大小與菌株有關， DH5α感受態細胞適合小于 15kb 的質粒。
Q：11802和11803有什么區別？
A:11802屬于普通速度轉化的感受態細胞，需要42℃熱激37℃孵育等步驟，而11803屬于快速轉化的感受態細胞，無需熱激孵育。
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