• 偶聯物HRP
• 經測試應用IHC-P,?WBmore details
• 種屬反應性
  與反應:?Human
  預測可用于:?Mouse, Rat
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tyrosine Hydroxylase. A synthetic peptide corresponding to residues surrounding serine 70 of human Tyrosine Hydroxylase.

• 陽性對照
  • WB: Human adrenal gland lysate. IHC: Human cerebellum tissue.
• 常規說明

  Produced using Abcam’s RabMAb^??technology. RabMAb^??technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-Tyrosine Hydroxylase antibody?[EP1533Y] (ab75875)

  Anti-Tyrosine Hydroxylase antibody (Alexa Fluor^??488)?[EP1533Y] (ab192463)

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
• 存儲溶液pH: 7.40
  Preservative: 0.1% Proclin
  Constituents: 30% Glycerol, 1% BSA, PBS
• ?
• 純度Immunogen affinity purified
• 克隆單克隆
• 克隆編號EP1533Y
• 同種型IgG
• 研究領域
  • Neuroscience
  ?
  • Neurotransmitter
  ?
  • Biogenic Amines
  ?
  • Dopamine

  • Neuroscience
  ?
  • Neurotransmitter
  ?
  • Biogenic Amines
  ?
  • Noradrenaline

  • Neuroscience
  ?
  • Cell Type Marker
  ?
  • Neuron marker
  ?
  • Soma marker

  • Cancer
  ?
  • Cancer Metabolism
  ?
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Cancer
  ?
  • Cancer Metabolism
  ?
  • Response to hypoxia

  • Metabolism
  ?
  • Pathways and Processes
  ?
  • Metabolism processes
  ?
  • Hypoxia

  • Metabolism
  ?
  • Pathways and Processes
  ?
  • Endocrine metabolism
  • Hormone biosynthesis

應用

靶標

• 功能Plays an important role in the physiology of adrenergic neurons.
• 組織特異性Mainly expressed in the brain and adrenal glands.
• 通路Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
• 疾病相關Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
  Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
• 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

• Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) 圖像

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) (ab193083)

    IHC image of Tyrosine Hydroxylase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193083 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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