重組人HVEM蛋白（Recombinant Human HVEM Protein），GMP級

產品貨號：GMP-TL722

產品描述（Description）

Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

分子別名（Synonym）

TNFRSF14、ATAR、HVEA、LIGHTR、TR2、CD270

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列編碼人 HVEM（ UniProtKB/Swiss-Prot:Q01973）表達帶有 Fc 標簽在 C 末端

分子量（Molecular Weight）

重組人HVEM蛋白 包含468個氨基酸，預測理論分子量為51.3 kD

純度（Purity）

≥95%采用 SDS-PAGE 凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定 LIGHT(His 標簽) at 5 μg/mL (100 μL/well) 能夠結合人 HVEM(Fc 標簽) 的線性范圍是 50-200ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融 

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Riccardo Sibilano, Nicolas Gaudenzio, Marianne K. DeGorter, Laurent L. Reber, Joseph D. Hernandez, Philipp M. Starkl, Oliwia W. Zurek, Mindy Tsai, Sonja Zahner, Stephen B. Montgomery, Axel Roers, Mitchell Kronenberg, Mang Yu, Stephen J. Galli Nat Commun. 2016; 7: 13696. Published online 2016 Dec 16. doi: 10.1038/ncomms13696 

2.、TNFRSF14 aberrations in follicular lymphoma increase clinically significant allogeneic T-cell responses Eleni Kotsiou, Jessica Okosun, Caroline Besley, Sameena Iqbal, Janet Matthews, Jude Fitzgibbon, John G. Gribben, Jeffrey K. Davies Blood. 2016 Jul 7; 128(1): 72–81. Prepublished online 2016 Apr 21. doi: 10.1182/blood-2015-10-679191

  注意：For research and manufacturing use.