DIG DNA 標記試劑盒
產(chǎn)品名稱: DIG DNA 標記試劑盒
英文名稱:
產(chǎn)品編號: 11175033910
產(chǎn)品價格: 0
產(chǎn)品產(chǎn)地: Roche
品牌商標: Roche
更新時間: null
使用范圍: null
北京鴻躍科技有限公司
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DIG DNA 標記試劑盒 DIG DNA Labeling Kit:Random primed DNA labeling with digoxigenin-dUTP, alkali-labile 用DIG-11-dUTP對DNA作隨機引物擴增法標記。帶DIG標記的探針可用于各種類型膜雜交,標記的探針通過酶聯(lián)免疫法檢測。 產(chǎn)品號 產(chǎn)品規(guī)格 廠商 價格 1175033 11175033910 Kit(40個標記反應):(40 labeling reactions) Roche 6545 DIG DNA Labeling Kit Application Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. The standard reaction with 1 g template DNA will give a yield of 260 ng DIG-labeled DNA after 1-hour incubation at 37°C, or 780 ng after 20-hour incubation. DIG-labeled DNA probes are used in all types of nucleic-acid filter hybridizations. DIG-labeled nucleic-acid hybrids are detected by an enzyme-linked immunoassay using anti-DIG-AP conjugate and chromogenic or chemiluminescence AP substrates. Product Description Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1 g digested human placenta DNA。 Contents 1. Unlabeled Control DNA 1, 20 l, [100 g/ml] 2. Unlabeled Control DNA 2, 20 l, [200 g/ml] 3. DNA Dilution Buffer, 2 x 1 ml 4. DIG-labeled Control DNA, 50 l, [5.2 g/ml] 5. Hexanucleotide Mix, 10x, 80 l 6. dNTP Labeling Mixture, 10x, 80 l 7. Klenow Enzyme, labeling grade, 40 l, [2 U/l]
