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Reagent :jetOPTIMUS DNA Transfection Reagent.

Molecule delivered :Plasmid DNA.

Applications :Transient and stable gene expression from plasmid DNA transfection.
CRISPR Genome editing using DNA approach.

Cell types :
Adherent hard-to-transfect mammalian cell:
- Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…).
- Stem cells (embryonic, mesenchymal, induced pluripotent).
- Cancer cell lines.

Number of transfections :1.5 ml of jetOPTIMUS transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol.

Storage :
Store jetOPTIMUS at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with :jetOPTIMUS Buffer.

Summary:
Ace your DNA transfection in the cell model you need!

As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).

Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI, jetPRIME, jetMESSENGER), Polyplus-transfection engineered an innovative delivery nanoparticle: jetOPTIMUS.

jetOPTIMUS is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.