Size??100ug
Cat code??200303
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??70kDa
Sensitivity??This antibody detects endogenous levels Hsp70(N-terminus) and does not cross-react with related proteins.
Immunogen??Purified recombinant human Hsp70(N-terminus) protein fragments expressed in E.coli.
UniProt Number??P08107
GeneBank ID??NM_005345
Subcellular location??Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
Gene_symbol??HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
Summary??This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In
conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates
the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the
ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located
in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode
similar proteins.
UniPort summary  Function??In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell
Application Images :??
Western blot detection of Hsp70(N-terminus) antibody in Heat shcok Hela,Hela&HepG2 lysates using Hsp70(N-terminus) antibody (1:1000 diluted).
Predicted band size: 70KDa
Observed band size: 70KDa.
Immunocytochemistry stain of Hela using HSPA1A antibody (1:300).