XPA Rabbit Polyclonal Antibody (KO Validated)-抗體-抗體-生物在線

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上海雅吉生物科技有限公司
XPA Rabbit Polyclonal Antibody (KO Validated)

XPA Rabbit Polyclonal Antibody (KO Validated)

商家詢價

產(chǎn)品名稱： XPA Rabbit Polyclonal Antibody (KO Validated)

英文名稱： XPA Rabbit Polyclonal Antibody (KO Validated)

產(chǎn)品編號： K5336

產(chǎn)品價格： null

產(chǎn)品產(chǎn)地： 上海

品牌商標(biāo)： 雅吉生物

更新時間： null

使用范圍： WB, IF, ICC

上海雅吉生物科技有限公司
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  • 地址 : 上海市閔行區(qū)元江路5500號第1幢5658室
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  • 所在區(qū)域 : 上海
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來源用途交叉反應(yīng)性理論分子量實(shí)際分子量
RabbitWB, IFH, M31kDa37kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實(shí)際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
WBIPIFIHCICCFCChIPChIP-seq
1:500-1:2000-1:50-1:100-----
抗體詳細(xì)信息如下::
About this Antibody
NameXPA Rabbit Polyclonal Antibody (KO Validated)
CategoryRabbit Polyclonal Antibody (pAb); Primary antibody
IsotypeIgG
Purification methodAffinity purification
Positive samplesHeLa, Jurkat, K-562, Mouse liver, Mouse kidney
Cellular locationNucleus
Customer validation-
About the Immunogen
ImmunogenRecombinant fusion protein of human XPA (NP_000371.1).
SequenceMAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM
Gene ID7507
Swiss ProtP23025
SynonymsXPA; XP1; XPAC; DNA repair protein complementing XP-A cells
CategoryDNA Damage and Repair
BackgroundNucleotide excision repair (NER) is a process by which cells identify and repair DNA lesions that result from chemical and radiation exposure. The DNA binding protein XPA is an essential part of a pre-incision complex that forms at sites of damage, and is necessary for the initiation of nucleotide excision repair. XPA is one of eight NER proteins (XPA-G, XPV) encoded by genes that are defective in cases of xeroderma pigmentosum, a disorder characterized by sensitivity to sunlight, predisposition to exposed tissue cancers, and neurological defects in some patients. Activation of XPA follows phosphorylation at Ser196 and results in increased NER activity. Phosphorylation of XPA at Ser196 is induced by UV exposure in an ATR-dependant fashion and promotes nuclear accumulation of XPA. Research studies suggest that XPA may be a direct substrate of the serine/threonine kinase ATR and that NER activity may be negatively regulated through dephosphorylation of Ser196 by the phosphatase WIP1.
包裝清單：
產(chǎn)品編號產(chǎn)品名稱包裝
AF5336XPA Rabbit Polyclonal Antibody (KO Validated)50μl
AZ050Western一抗稀釋液50ml
—說明書1份
保存條件：
XPA Rabbit Polyclonal Antibody (KO Validated) -20oC保存，Western一抗稀釋液-20oC或4oC保存，一年有效。Western一抗稀釋液優(yōu)先推薦4oC保存，長期不使用可以考慮-20oC保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
代表性圖片：
Fig. 1. Western blot analysis of extracts of various cell lines, using XPA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: QuickBlock? Blocking Buffer (P0231).Fig. 2. Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: QuickBlock? Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 3. Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.Fig. 4. Immunofluorescence analysis of A549 cells using XPA antibody.
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