Anti-BRaf Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術有限公司
Anti-BRaf Mouse Monoclonal Antibody

Anti-BRaf Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-BRaf Mouse Monoclonal Antibody

英文名稱： Anti-B-Raf Mouse Monoclonal Antibody

產品編號： 26129

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC、IP

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
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  • 郵箱 : neweast3@126.com

B-Raf [26129]
[100 μL]		 ?

Catalog Number:??26129
Gene Symbol:??B-Raf
Description: ??Anti-B-Raf Mouse Monoclonal Antibody
Background: ??B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen: ??A synthetic peptide from the internal region of human B-Raf.
Applications: ??ELISA, WB, IHC, IP
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:100-1:1000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Anti-B-Raf antibody recognizes B-Raf of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles
?
Western blot:??
Western blot analysis of recombinant B-Raf protein. Purified his-tagged B-Raf protein (Cat. #10161) was blotted with anti-B-Raf monoclonal antibody (Cat. #26129).

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Publications:
1.??Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
????Mod Pathol. 2013 Mar;26(3):414-20.
2.??Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
????Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.??Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
????human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.??Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
????human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.??BRAF V600E Mutation-Specific Antibody: A Review
????Seminars in Diagnostic Pathology