Size??100ug
Cat code??200130
Species Cross-reactivity??H,M
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??null
Isotype??IgG2b
Molecular Weight??50kDa
Sensitivity??This antibody detects endogenous levels of SMYD2 and does not cross-react with related proteins.
Immunogen??Purified recombinant human SMYD2 protein fragments expressed in E.coli.
UniProt Number??Q9NRG4
GeneBank ID??NM_020197
Subcellular location??Cytoplasm ? cytosol；Nucleus
Formulation??ascites
Altername??HSKM-B；Histone methyltransferase SMYD2，EC=2.1.1.43；Lysine N-methyltransferase 3C；SET and MYND domain-containing protein 2
Gene_symbol??KMT3C; HSKM-B; ZMYND14
Summary??SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
UniPort summary  Function??Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 ′Lys-4′ (H3K4me) and dimethylates histone H3 ′Lys-36′ (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates ′Lys-370′ of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates ′Lys-860′ of RB1/RB.
Application Images :??

Western blot detection of SMYD2 in 3T3,HT1080，A431&Jurkat cell lysates and  using SMYD2 antibody (1:1000 diluted). Predicted band size: 50KDa Observed band size: 50KDa