產品簡介
英文名稱
Anti-ALYREF Polyclonal Antibody
宿主
Rabbit
別名
ALY;ALY/REF;BEF;REF;THOC4;THO complex subunit 4
稀釋比例
WB 1:5000-8000. IHC 1:100-300.
蛋白分子量
27kDa
Gene ID
10189
保存
Store at -20°C. Avoid freeze / thaw cycles.
儲存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
純化方法
Affinity purification
亞型
IgG
免疫原
A synthetic peptide of human ALYREF
亞細胞定位
Nucleus Cytoplasm
Swiss Prot
Q86V81
背景資料
Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.
Public Immunogen Range
207-257/257aa
克隆類型
Polyclonal Antibody
交叉反應
Human Rat Mouse
應用
WB IHC
規格
50ul 100ul
備注：
以上數據均來自公開文獻, Solarbio暫未進行獨立驗證, 僅供參考。
These protocols are for reference only. Solarbio does not independently validate these methods.
實驗圖
Western blot analysis with ALYREF antibody diluted at 1:6000;Lane: HepG2,Hela,PC-3M-IE8,Mouse testis,Mouse spleen,Mouse thymus,Rat brain
Immunohistochemistry?of?paraffin-embedded Human thyroid cancer?with?ALYREF antibody diluted at