基本信息

產品名稱Recombinant Human IGF-1 Protein英文名稱Recombinant Human IGF-1 Protein規格或純度Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 49-188aa生物活性Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED?? for this effect is typically <4.6 ng/mL.內毒素水平<0.2 EU/μg表達系統Pichia pastoris種屬Human氨基酸49 to 118序列GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA蛋白標簽No tagN-末端序列Gly蛋白長度Full length proteinCarrier FreeYesAnimalFreeYesAccession#P05019來源Recombinant預測分子量7.7 KD±0.8kDaSDS-PAGE8.5 kDa, under reducing conditions; 8.5 kDa, under non-reducing conditions.

相關屬性

特點與好處·Application:Cell Culture,FuncS,HPLC,MS,SDS-PAGE物理外觀Lyophilized儲存緩沖液10mM磷酸鈉緩沖液復溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a儲存溫度-20°C儲存運輸條件超低溫冰袋運輸穩定性與儲存Stable at -20℃ to 24 months, stable at 2-8 ℃ to 4 weeks. Prepared as 100μg/ml storage solution, 2~8℃ can be stably stored for a week.

常規信息

細胞定位Secreted.序列相似性Belongs to the insulin family.涉及疾病Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.基因別名IGF, IGF-I, IGFI, MGF英文別名IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Soma別名IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Soma

一般描述

產品描述：

無動物源性：無動物病毒、無致病物質、無外源因子污染，安全性高。

胰島素樣生長因子-1(IGF-1)又稱生長因子 C (SomatamedinC)，屬于胰島素 樣生長因子家族，在結構上與胰島素原具有同源性。IGF-1 是一種促有絲分列的多肽生長因子，在體外刺激肌肉、骨、軟骨等多種細胞的增殖和存活。IGF-1 能與 IGF-1R、IGF-2R 和胰島素受體結合，喚醒多種信號通路，參與細胞的增殖、分化、存活、 生長、凋亡和再生。重組人胰島素樣生長因子(IGF-1)由 70 個氨基酸組成，是一種用于細胞培養的生物活性蛋白。

本品系重組酵母菌表達、經多步分離純化，再經 0.22μm 濾膜過濾除菌后冷凍干燥而制成的凍干粉。

1.避免在高溫環境長時間存放；操作過程中應避免污染。

2.僅供研究使用，不得用于動物或人類的診斷或治療。

生物活性驗證方法：利用 IGF-1 對人乳腺癌細胞株 MCF-7 的刺激作用，測定其生物學活性。

產品應用：

廣泛用于細胞培養，可增強細胞增殖、細胞生長和細胞分化。

Purity

≥ 95% SDS-PAGE.

Molecular weight informat

M - 2.84 Da (Calculated mass 7711.84 Da)GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA

Additional sequence information

N-terminal glycineFull-length mature chain lacking the signal peptide and both pro-peptides.

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

Product description:

Animal origin-Free; Animal viruses-Free; Pathogenic substances-Free; Exogenous factor pollution, High safety.

Insulin-like growth factor-1 (IGF-1), also known as growth factor C (SomatamedinC), belongs to the insulin-like growth factor family and has homology with proinsulin in structure. IGF-1 is a peptide growth factor that promotes mitosis, and stimulates the proliferation and survival of various cells such as muscle, bone, and cartilage in vitro. IGF-1 can bind to IGF-1R, IGF-2R and insulin receptors, wake up a variety of signaling pathways, and participate in cell proliferation, differentiation, survival, growth, apoptosis and regeneration. Recombinant human insulin-like growth factor (IGF-1) consists of 70 amino acids and is a bioactive protein used in cell culture.

This lyophilized powder is made of recombinant yeast expression, multi-step separation and purification, and then 0.22μm membrane filter sterilization and freeze-drying.

Product application：

Widely used in cell culture to enhance cell proliferation, cell growth and cell differentiation.

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Protein Bioactivity: Recombinant Human IGF-1 Protein (rp147252)

Recombinant Human IGF-1 Protein (rp147252)-Protein Bioactivity

Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED?? for this effect is typically <4.6ng/mL.

SDS-PAGE: Recombinant Human IGF-1 Protein (rp147252)

Recombinant Human IGF-1 Protein (rp147252)-SDS-PAGE

5μg/lane of Recombinant Human IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 8.5 kDa.