description;for research or for further manufacturing use;without L-glutamine.
sterility;sterile-filtered.
form;liquid.
technique(s);cell culture | mammalian: suitable.
components;phenol red: yes;glucose: 3.45 g/L;L-glutamine: no;HEPES: 7.5 mM;NaHCO3: 1.6 g/L.
Application;EX-CELL 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media (Catalog No. 14324C and 14326C) can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System, and DHFR- selection systems).