Applicable For:Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.
Elution Volume:≥ 6 l of DNA Elution Buffer.
Equipment:Microcentrifuge.
Purity:A260/A280 >1.8, A260/A230>1.8.
Sample Source:DNA excised from TAE/TBE-buffered agarose gels.
Size Range:50 bp to 23 kb.
Yield:Typically, up to 5 g total DNA per column can be eluted with ≥6 l water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
HIGHLIGHTS:
Quick recovery of ultra-pure DNA from agarose gels.
Column design permits DNA elution at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.