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Product: Anti-Fas Ligand, clone H11
Cat. No.: MC-106 (100 μg)
Background:
Fas Ligand (FasL) is a type II membrane protein
and a member of the tumor necrosis factor
family. FasL is released by cleavage by
metalloproteinases. When interacting with the
Fas receptor, the trimeric FasL induces
apoptosis. It is responsible for the activationinduced
cell death of peripheral T cells and
functions as an effector molecule for cytotoxic T
cells. FasL is expressed in activated
lymphocytes, Sertoli cells, and in certain tumor
cells.
Species Reactivity:
Mouse. Others not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
Synthetic peptide corresponding to aa 196-220
of the mouse Fas Ligand protein (CD95L; APO-
1L; CD178).
Format:
100 μL of 1 mg/mL antibody in PBS with 0.02%
sodium azide.
Purity:
> 95% by SDS-PAGE.
Storage:
Store undiluted at 4°C. Do not freeze/thaw.
Applications and Suggested Dilutions:

Immunocytochemistry: Use anti-Fas Ligand,
clone H11 unlabeled (Cat. No. MC-106),
biotin-labeled (Cat. No. MC-126), or FITClabeled
(Cat. No. MC-136).

Flow Cytometry: Use Anti-Fas Ligand, clone
H11 unlabeled (Cat. No. MC-106), biotinlabeled
(Cat. No. MC-126), or FITC-labeled
(Cat. No. MC-136). [Note: Activated T cells do
not express high levels of surface Fas Ligand.]

Western Blot: Excellent for this application.
Use 2 μg/mL. Use unlabeled (Cat. No. MC-
106) or biotin-labeled (Cat. No. MC-126).
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1. Immunocytochemistry: Detection of FasL in 293T cells
transfected with a FasL expression vector.
Method: Transfected cells were plated onto polylysine treated glass
slides, fixed and permeabilized in methanol at –20°C for 5 min, then
in acetone at –20°C for 30 sec. After 3 washes in PBS, 0.1% BSA,
slides were incubated for 1 hr at RT with 20 μg/mL of biotinylated
H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS,
FITC-conjugated streptavidin was added for 30 min. Slides were
washed again in PBS and visualized using a fluorescence
microscope.
Method: FasL transfected 293T cells (5x105) were incubated on ice
for 30 min in 50 μL FACS buffer (PBS, 5% fetal calf serum, 0.02%
sodium azide) containing 1 μg of FITC-labeled H11 antibody (MC-
136). After washing in FACS buffer, cells were analyzed by flow
cytometry. Do not exclude pre-apoptotic cells during data acquisition.
Limitations:
For in vitro research use only. Not for use in diagnostics or
in humans.
Warranty:
No warranties, expressed or implied, are made regarding the
use of this product. KAMIYA BIOMEDICAL COMPANY is not
liable for any damage, personal injury, or economic loss
caused by this product.