Active Gαi GTP [CR-M26901]

[30 µL]

Active Gαi GTP [CR-M26901] [30 µL] Catalog Number:  CR-M26901 Gene Symbol:  Gnai Description:   Anti Active Gαi Mouse Monoclonal Antibody Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored. Immunogen:   Recombinant full length protein of active Gαi1 Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;                                                     1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG1 Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Gαi antibody recognizes active Gαi1, Gαi2, and Gαi3 of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:

Gαi activation assay.  A. CHO cells were  transfected with wild-type Gαi1 (lanes 1 and 2) or constitutively  active Gαi1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS  (lane 3). Lysates were then incubated with an anti-active Gαi monoclonal antibody  (Cat. No.CR-M26901) (top panel). The precipitated active Gαi was immunoblotted with  an anti Gαi monoclonal antibody (Cat. No. CR-M26003). The bottom panel shows the  Western blot with anti Gαi monoclonal antibody (Cat.No. CR-M26003) of the cell lysates.  B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or  without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gαi monoclonal antibody  (Cat. No. CR-M26901) (top panel).The precipitated active Ga i was immunoblotted with an anti Gαi rabbit polyclonal  antibody (Cat. No. CR-R21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.

Publications: 1.  Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells     Biochem J. 2013 Feb 15;450(1):209-19 2.  CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor     J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61 3.  CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma     Cancer Res. 2012 Jan 1;72(1):122-32 4.  Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts     Endocrinology. 2012 Oct;153(10):4918-28 5.  WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins     Cell Signal. 2011 Mar;23(3):550-4 6.  Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis     Biochim Biophys Acta. 2011 Mar;1813(3):466-72 7.  Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I     Steroids Volume 77, Issue 5, April 2012, Pages 424–432 8.  Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits     J Biol Chem. 2010 Aug 27;285(35):26976-86 9.  Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7     Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32 10.  Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin     Mol Biol Cell. 2014 Nov 5;25(22):3654-71 11.  Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan     Mol Cell Biol. 2014 Nov 15;34(22):4186-99 12.  Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain J Biol Chem. 2014 May 2;289(18):12748-58