踝蛋白抗體
產品名稱： 踝蛋白抗體
英文名稱： Talin
產品編號： 3619
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： ELISA IHC-P IHC-F IF
上海雅吉生物科技有限公司
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- 地址 : 上海市閔行區元江路5500號第1幢5658室
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- 所在區域 : 上海
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- 郵箱 : yajikit@163.com
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| 中文名稱 | 踝蛋白抗體 |
| 別????名 | TLN 1; ILWEQ; Talin 1; TLN; TLN1; TLN1_HUMAN.?? |
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| 研究領域 | 細胞生物??免疫學??轉錄調節因子??細胞粘附分子?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,?Rat,? (predicted: Human,?Dog,?Pig,?Cow,?Horse,?Rabbit,?) |
| 產品應用 | ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 280kDa |
| 細胞定位 | 細胞漿?細胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Talin:1601-1750/2541? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Subunit: Binds with high affinity to VCL and with low affinity to integrins. Interacts with PTK2/FAK1 (By similarity). Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM. Subcellular Location: Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Colocalizes with LAYN at the membrane ruffles. Similarity: FERM domain. Contains 1 I/LWEQ domain. SWISS: Q9Y490 Gene ID: 7094 Database links: Entrez Gene: 395194?Chicken Entrez Gene: 7094?Human Entrez Gene: 21894?Mouse Entrez Gene: 313494?Rat Omim: 186745?Human SwissProt: P54939?Chicken SwissProt: Q9Y490?Human SwissProt: P26039?Mouse Unigene: 471014?Human Unigene: 208601?Mouse Unigene: 7715?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. TALIN-踝蛋白。是重要的細胞骨架肌動蛋白結合蛋白，由兩個270kDa的亞單位組成的反向 平行的同源二聚體，與整合蛋白B尾部的結合誘導了整合蛋白胞外結構域構象的改變,起到了對整合蛋白的激活。 |
| 產品圖片 |  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Talin) Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Talin) Polyclonal Antibody, Unconjugated (bs-3619R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse fetal liver;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Talin Polyclonal Antibody, FITC conjugated(bs-3619R-FITC) 1:200, at 1:200 dilution for 60 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei  Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Talin Polyclonal Antibody, FITC conjugated(bs-3619R-FITC) 1:200, dilution for 60 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
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