T淋巴細胞激活蛋白抗體-抗體-抗體-生物在線
上海雅吉生物科技有限公司
T淋巴細胞激活蛋白抗體

T淋巴細胞激活蛋白抗體

商家詢價

產品名稱： T淋巴細胞激活蛋白抗體

英文名稱： Sca1

產品編號： 3752

產品價格： null

產品產地： 上海

品牌商標： 雅吉

更新時間： null

使用范圍： ELISA IHC-P IHC-F Flow-Cyt IF

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中文名稱 T淋巴細胞激活蛋白抗體
別????名 Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; Sca1 / Ly6A/E; SCA-1; T-cell-activating protein; TAP; Ly6al; Ly6a; LY6A_MOUSE.??
研究領域 細胞生物??免疫學??細胞膜受體??細胞表面分子??t-淋巴細胞??b-淋巴細胞??
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,?Mouse,?Rat,?
產品應用 ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=2μg /Test?IF=1:100-500?（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量 9kDa
細胞定位 細胞膜?
性????狀 Liquid
濃????度 1mg/ml
免?疫?原 KLH conjugated synthetic peptide derived from mouse Sca1/Ly6A/E:15-110/134?
亞????型 IgG
純化方法 affinity purified by Protein A
儲?存?液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.

Function:
T-cell activation.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
O-glycosylated. Not N-glycosylated.
Not phosphorylated.

Similarity:
Contains 1 UPAR/Ly6 domain.

SWISS:
P05533

Gene ID:
110454

Database links:

Entrez Gene: 110454?Mouse

SwissProt: P05533?Mouse

Unigene: 425949?Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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產品圖片 Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Tissue/cell: mouse thymus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control: Mouse Thymus Cells(blue).
Primary Antibody: Rabbit Anti-Sca1/FITC Conjugated antibody (bs-3752R/FITC), Dilution: 2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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