工作原理

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• 即用型

• 快速

• 靈敏

• 適用于3D細胞培養

• 成本低

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本試劑盒使用吖啶橙（AO）和碘化丙啶（PI）兩種核染色（核酸結合）染料。AO可滲透活細胞和死細胞，可染色所有有核細胞產生綠色熒光；PI只穿透細胞膜受損的有核細胞的細胞膜，并染色死亡細胞產生紅色熒光。由于猝滅作用，當細胞同時被AO和PI染色時，所有活的有核細胞產生綠色熒光，所有死的有核細胞產生紅色熒光。

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工作方法

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Data and References

Figure 1. Live-dead cell viability analysis by using Cyto3D Live-Dead Assay Kit.
Glioblastoma cells (SF 298, about 60% cell viability) were 3D cultured in VitroGel system for 2 days. 2 μL of Cyto3D reagent was added to each well containing 50 μL hydrogel and 50 μL cover medium.? The mixture was incubated at 37 °C for 5-10 min. The cells were then observed under a fluorescent microscope. The images show the Live (green) and Dead (orange) cells within the 3D hydrogel matrix. The z-stack images of cells within hydrogel were then 3D reconstructed and showed in the 4D view images. The live and dead cells at higher levels of the hydrogel clearly show in the images by using Cyto3D Live-Dead Assay Kit.

Figure 2. Live-dead cell viability images of stem cell spheroids.
Stem cells were static suspension-cultured in VitroGel STEM (CAT# VHM02) for 5 days. 2 μL of Cyto3D reagent was added to each well containing 100 μL cell suspension.? The mixture was incubated at 37 °C for 5-10 min. The cells were then observed under a fluorescent microscope. The images show the Live (green) and Dead (orange) stem cell spheroids cultured in a 3D hydrogel matrix. The live-dead dyes of Cyto3D Live-Dead Assay Kit can successfully penetrate into large cell spheroids for cell viability analysis.