Recombinant Human PDGF-AA
 
Catalog Number:
AF-100-13A
Synonyms:
Platelet-Derived Growth Factor-AA, ?Glioma-derived growth factor (GDGF), ?Osteosarcoma-derived Growth Factor (ODGF)
Description:
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Manufactured using all non-animal reagents.
AA Sequence:
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The?ED50?as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106?units/mg.
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Cross Reactivity:
Dog, Monkey, Mouse, Rat
Research Interests:
Angiogenesis/ Cardiovascular
Bone, Skeletal, Cartilage
Cancer
Immune System
Neurobiology
Stem Cells & Differentiation
Wound Healing
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Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.