abcam兔單克隆抗體Anti-Vimentin抗體[EPR3776] - Cytoskeleton Marker-抗體-抗體-生物在線

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深圳市宇德立生物科技有限公司
abcam兔單克隆抗體Anti-Vimentin抗體[EPR3776] - Cytoskeleton Marker

abcam兔單克隆抗體Anti-Vimentin抗體[EPR3776] - Cytoskeleton Marker

商家詢價

產品名稱： abcam兔單克隆抗體Anti-Vimentin抗體[EPR3776] - Cytoskeleton Marker

英文名稱： CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody

產品編號： ab92547

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯系人 :
  • 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
  • 郵編 :
  • 所在區域 : 廣東
  • 電話 : 133****4454 點擊查看
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  • 郵箱 : 1484332550@qq.com

免疫原：Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Vimentin aa 400 to the C-terminus (acetyl ). The exact sequence is proprietary.

常規說明

This product is a recombinant rabbit monoclonal antibody.

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We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

應用

Our?Abpromise guarantee?covers the use of?ab92547?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
ICC/IF ? 1/250.
WB ? 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
Flow Cyt ? 1/50.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P ? 1/200 - 1/500.

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靶標

  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相關Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 結構域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻譯后修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker 圖像

    • Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/20000 dilution (purified) + COS-1 cell lysate at 20 μg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size :?54 kDa
      Observed band size :?54 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST