• 靶標種屬Mouse
• 經測試應用ICC/IF,?IP,?Dot Blot,?ELISA,?IHC-P,?IHC-Fr,?Immunomicroscopy,?WBmore details
• 免疫原

  mouse IgG whole molecule

• 偶聯物HRP

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C.
• 存儲溶液pH: 7.2
  Preservative: 0.01% Gentamicin sulphate
  Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
• 濃度1 mg 濃度為 2 mg/ml?
  
• 純度IgG fraction
• 純化說明This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• 共軛說明Horseradish Peroxidase (HRP)
• 克隆多克隆
• 同種型IgG
• 常規說明The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
• 研究領域
  • Immunology
  ?
  • Immunoglobulins
  ?
  • Heavy Chain
  ?
  • IgG

  • Secondary antibodies
  ?
  • anti-Mouse
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Goat Anti-Mouse IgG H&L (HRP) 圖像

• 

  Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time :?1 minute

  This image is courtesy of an anonymous Abreview.

  See Abreview

• 

  Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

  ab49969?Immunoprecipitating?HA Tag?in human?293T whole cell lysate. 25μg of cell lysate was incubated with primary antibody (1/2500 in?lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting?a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

  IHC image of?beta actin?staining in?human colon?formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789,?1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/500 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
  Ab6789 was used at dilution 1/10000 with the primary antibody?ab55504?in WB. See the review on?ab55504.