SPECIFICATION SHEET

Catalog #:???????????????????????? L1C00501?????????????????? Lot #:??????? C080301

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Description: ?????????????????? MAb to Neuron Specific Enolase

??????????????????????????????????? Monoclonal Antibody to Neuron Specific Enolase (NSE)

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Specificity:?????????????????????? Gamma enolase.? Specific for the gamma subunit without detectable cross-reactivity with the alpha or beta subunits.? Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.?

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Clone:???????????????????????????????? ML01N001

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Host Animal:????????????????? Mouse.? Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.??????????

Isotype: ??????????????????????????? IgG_2a

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Source:?????????????????????????????? Ascites

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Immunogen:?? ???????????? Purified neuron-specific enolase from human brain

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Format:????????????????????????????? Purified, Liquid

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Purification:? ??????????????? >95% pure (SDS-PAGE).? Protein G chromatography

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Concentration: ??????????? 2 mg/ml (OD280nm, E^0.1% = 1.4)

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Affinity Constant:? ?? 1x10^-9

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Buffer:?????????????????????????????? PBS, pH 7.4

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Preservative: ???????????????? None

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Applications: ??????????????? Suitable for use in ELISA.? May be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections.? Each laboratory should determine an optimum working titer for use in its particular application.? Other applications have not been tested but use in such assays should not necessarily be excluded.

?????????????????????????????????????????????? Recommended pairs in ELISA:

??????????????????????????????????????????????? Capture???????????????????????????????? Detection

??????????????????????????????????????????????? L1C00501??????????????????????????? L1C00502

Storage:???????????????????????????? Short-term (up to 2 months) store at < –20°C.? Long term (>2 months) store at <–40°C.