A rapid method for gene expression analysis, PURExpress is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
Control amino acids and tRNA are provided separately.
Ideal for studies requiring control of the concentration or content of amino acids and/or tRNAs in a translation reaction.
Applications include non-natural amino acid incorporation, amino acid tRNA synthetase studies and labeling with amino acids that contain stable isotopes.